Metal ion requirement for OM hydrolysis by various proteins in human plasma
Percentages were determined as the percentage activity in comparison with values measured in the presence of Ca2+ ions. The substrate concentration was 10 μM.
| Enzyme Source | +Ca2+ | +Mg2+ | +Zn2+ |
|---|---|---|---|
| nmol · min−1 · mg protein−1 (% activity) | |||
| Plasma | 7.91 (100) | 0.266 (3.4) | 0.0918 (1.2) |
| Recombinant PON1192QQ | 9.50 (100) | 0.650 (6.8) | 0.750 (7.9) |
| Recombinant PON1192RR | 20.6 (100) | 2.25 (10.9) | 1.75 (8.5) |
| Serum albumin | 0.0102 (100) | 0.0106 (103.9) | 0.0164 (160.8) |