TABLE 1

Initial uptake and biliary clearance of test compounds in day 5 SCHH

After a preincubation with 1 mM ABT for 30 min, the test compounds were incubated in the presence or absence of ABT in day 5 SCHH (lot Hu4168). Data represent results from single studies run in duplicate. A minimum of two experiments were performed on different days to verify a CV% <15%.

CompoundInhibitorUptake CLBiliary CL
μl · min1 · mg protein1
Rosuvastatin (0.2 μM)Control6.71.6
+ABT6.62.2
Atorvastatin (0.2 μM)Control121.4
+ABT101.3
Midazolam (1 μM)Control130
+ABT140
  • CL, clearance.