The reaction conditions for P450 enzyme assay using the liver microsomes
| P450 | Substrate | Metabolite | Substrate Concentration | Microsomal Concentration | Reaction Time |
|---|---|---|---|---|---|
| μM | mg/ml | min | |||
| CYP1A2 | Phenacetin | Acetaminophen | 5 | 0.5 | 20 |
| CYP2B6 | Bupropion | Hydroxybupropion | 5 | 0.5 | 20 |
| CYP2C8 | Rosiglitazone | N-Demethylrosiglitazone | 2 | 0.2 | 20 |
| 5-Hydroxyrosiglitazone | |||||
| CYP2C9 | (S)-Warfarin | 7-Hydroxywarfarin | 4 | 1 | 30 |
| Diclofenac | 4′-Hydroxydiclofenac | 4 | 0.2 | 30 | |
| CYP2C19 | (S)-(−)-Mephenytoin | 4′-Hydroxymephenytoin | 20 | 0.5 | 20 |
| CYP2D6 | Dextromethorphan | Dextrorphan | 1 | 0.2 | 20 |
| CYP3A4 | Triazolam | 1′-Hydroxytriazolam | 2 | 0.2 | 20 |
| 4-Hydroxytriazolam |