Quantification of OATP1B1, OATP1B3, and OATP2B1 in suspension hepatocytes and SCHHs of lot Hu4165
The protein expression of OATP1B1, OATP1B3, and OATP2B1 in SCHHs was measured by LC-MS/MS and compared with that in suspension hepatocytes.
| Suspension | SCHH at Day 5 | Change of Suspension | |
|---|---|---|---|
| fmol/μg protein | % | ||
| OATP1B1 | 3.42 ± 0.11 | 5.28 ± 0.22 | 154* |
| OATP1B3 | 1.50 ± 0.15 | 0.88 ± 0.11 | 59* |
| OATP2B1 | 1.84 ± 0.15 | 1.23 ± 0.12 | 67* |
↵* P < 0.05, compared with suspension.