Relay assay conditions

Assay ParametersAssay Conditions
Test compound concentration1 μM
Hepatocyte cell density0.5 million cells/ml
Organic solvent contentDMSO of 0.025%, MeOH of 0.125%
Incubation volume0.5 ml
Supernatant transfer volume0.3 ml
Plate format24-well
Incubation temperature37°C
Incubation conditions95% O2/5% CO2/75% RH
Incubation time4 h/relay
Standard number of relays5 relays
  • DMSO, dimethyl sulfoxide; MeOH, methanol; RH, relative humidity.