Assay Parameters | Assay Conditions |
---|---|
Test compound concentration | 1 μM |
Hepatocyte cell density | 0.5 million cells/ml |
Organic solvent content | DMSO of 0.025%, MeOH of 0.125% |
Incubation volume | 0.5 ml |
Supernatant transfer volume | 0.3 ml |
Plate format | 24-well |
Incubation temperature | 37°C |
Incubation conditions | 95% O2/5% CO2/75% RH |
Incubation time | 4 h/relay |
Standard number of relays | 5 relays |
DMSO, dimethyl sulfoxide; MeOH, methanol; RH, relative humidity.