Incubation conditions
| P450 | Substrate | Metabolite | HLM | Incubation Time |
|---|---|---|---|---|
| mg/ml | min | |||
| 1A2 | 50 μM phenacetin | Acetaminophen | 0.05 | 30 |
| 2A6 | 1 μM coumarin | 7-Hydroxycoumarin | 0.05 | 10 |
| 2B6 | 75 μM bupropion | hydroxybupropion | 0.1 | 15 |
| 2C8 | 10 μM paclitaxel | 6α-Hydroxypaclitaxel | 0.1 | 15 |
| 2C9 | 100 μM tolbutamide | Hydroxytolbutamide | 0.1 | 20 |
| 2C19 | 50 μM (S)-mephenytoin | 4′-hydroxymephenytoin | 0.2 | 30 |
| 2D6 | 5 μM dextromethorphan | Dextrorphan | 0.05 | 10 |
| 2E1 | 100 μM chlorzoxazone | 6-Hydroxychlorzoxazone | 0.1 | 10 |
| 3A | 5 μM midazolam | 1′-Hydroxymidazolam | 0.025 | 5 |
| 3A | 75 μM testosterone | 6β-Hydroxytestosterone | 0.05 | 10 |