TABLE 3

Glucuronidation activity of the three different UGT1A1 constructs and HLM

The rate values are means ± S.D. (n = 3). The relative expression values of the different UGT1A1 variants and HLM (Fig. 3A) are indicated in parentheses.

Rate (pmol · min1 · mg1)Substrate1A1-TL (1.2)1A1-His (2.4)1A1-XHC (1.0)HLM (1.01)
Actual1-Naphthol94.8 ± 1.9109 ± 4.8104 ± 0.6
4-MU80.3 ± 2.7112 ± 1896.6 ± 3.0
Ethinylestradiol71.7 ± 6.095.9 ± 5.390.3 ± 4.8100.6 ± 5.1
Normalized1-Naphthol80.4 ± 1.645.0 ± 2.0**104 ± 0.6**
4-MU68.0 ± 2.346.5 ± 7.4*96.6 ± 3.0**
Ethinylestradiol60.8 ± 5.139.6 ± 2.2*90.3 ± 4.8*99.6 ± 5.1**
  • The significance of the difference (either lower or higher) in the normalized rates between the -His and -XHC variants to the results with the -TL variant is indicated (p > 0.05): * p > 0.01; ** p > 0.001.