TABLE 2

Relative abundance of metabolites formed from lapatinib (50 μM) incubated with NADPH-fortified rCYP3A4 and rCYP3A5 (100 pmol/ml) for 30 minutes, followed by ultracentrifugation

IdentityRetention Time Relative to LapatinibExperimental m/zaCYP3A4:CYP3A5CYP3A5:CYP3A4
%
M10.50473.105255.4180.5
M20.78489.099546.0217.2
M30.95597.136786.0116.3
M40.97475.1336225.244.4
M51.10613.133180.7123.9
M61.11595.1220239.541.8
M71.13476.1176261.538.2
M81.14597.1368140.671.1
M91.18489.1125389.225.7
M101.21474.1016124.880.1
  • a Accurate m/z values and peak areas taken from the low energy MSE data set.