Primers used to test for alternative AKR1D1 mRNA transcripts
| Primer | Sequence (5′ → 3′) | Location in mRNA | Length |
|---|---|---|---|
| bp | |||
| AKR-FP | CCCTAGGACACCTTTCTA | 5′ UTR | 1353 |
| AKR-RP | TCATATGTTGCCTTTGATG | 3′ UTR | |
| AKR-FPN | ATGGATCTCAGTGCTGCAAGT | Start of Exon 1 | 989 |
| AKR-RPN | CCCTGCAGTCAGTATTCATCA | Exon 9/3′ UTR | |
| AKR-FP1 | ACCAGGTTGAGTGCCAT | Exon 5/Exon 6 | 711 |
| AKR-RP | TCATATGTTGCCTTTGATG | 3′ UTR | |
| AKR-FP1 | AACCAGGTTGAGTGCCAT | Exon 5/Exon 6 | 416 |
| AKR-RPN | CCCTGCAGTCAGTATTCATCA | Exon 9/3′ UTR |