In vitro alkaline phosphatase-mediated half-life and normalized rate of metabolism rate of various phosphate prodrugs
Data are reported as the mean of triplicates (CV < 10%).
| Enzyme | Enzyme Conc. | t1/2 (Normalized Rate of Metabolism) | ||||
|---|---|---|---|---|---|---|
| Dexamethasone Phosphate | Fosphenytoin | Clindamycin Phosphate | Ritonavir Oxymethyl Phosphate | Ritonavir Phosphate | ||
| IU/ml | min (rate/protein concentration: ml/IU per minute) | |||||
| Rat rIALP | 0.03 | 1.7 (13.6) | 0.7 (33.0) | 11.5 (2.0) | 13.1 (1.8) | >120 (<0.19) |
| RIMS | 0.0185 | 3.2 (11.7) | 1.3 (28.8) | 66.3 (0.56) | 36.9 (1.0) | >120 (<0.19) |
| Monkey rIALP | 0.0045 | 6.6 (23.3) | 2.2 (70.0) | 120 (1.3) | 11.6 (13.3) | >120 (<0.19) |
| Human placental ALP | 0.03 | 1.5 (15.4) | 1.4 (16.5) | 9.6 (2.4) | 14.6 (1.6) | >120 (<0.19) |
| Bovine ALP | 0.03 | 8.3 (2.8) | 6.2 (3.7) | 17.1 (1.4) | 5.0 (4.6) | >120 (<0.19) |