Fold-increase of purpurin-18 fluorescence with inhibitors
| Human ABCG2 | Mouse ABCG2 | ||||
|---|---|---|---|---|---|
| H460 MX20 | MCF FLV500 | 3T3 MX15 | Ltk-HoeR415 | ||
| Inhibitor | Conc. | Folda ± S.D. | |||
| μM | |||||
| Ko143 | 10 | 9.00 ± 0.03 | 5.00 ± 0.04 | 2.89 ± 0.01 | 5.69 ± 0.01 |
| Fumitremorgin C | 10 | 8.57 ± 0.03 | 7.64 ± 0.03 | 3.45 ± 0.01 | 4.86 ± 0.01 |
| Elacridar | 5 | 4.62 ± 0.02 | 3.38 ± 0.02 | 1.96 ± 0.01 | 3.73 ± 0.04 |
| Tariquidar | 5 | 5.46 ± 0.04 | 4.23 ± 0.04 | 1.74 ± 0.01 | 4.10 ± 0.02 |
| Nilotinib | 5 | 6.04 ± 0.01 | 3.88 ± 0.06 | 2.69 ± 0.13 | 4.77 ± 0.01 |
| Quercetin | 50 | 6.03 ± 0.04 | 5.03 ± 0.04 | 1.53 ± 0.01 | 2.59 ± 0.02 |
| NSC73306 | 100 | 5.10 ± 0.02 | 3.78 ± 0.05 | 2.04 ± 0.01 | 3.27 ± 0.01 |
| Flavoperidol | 5 | 4.09 ± 0.03 | 1.85 ± 0.02 | 1.84 ± 0.02 | 3.90 ± 0.02 |
| Chrysin | 50 | 15.11 ± 0.11 | 8.73 ± 0.07 | 4.49 ± 0.02 | 9.49 ± 0.44 |
| Benzoflavone | 5 | 8.80 ± 0.02 | 6.34 ± 0.02 | 4.45 ± 0.09 | 10.30 ± 0.04 |
| Estradiol | 100 | 8.50 ± 0.02 | 6.11 ± 0.12 | 3.37 ± 0.03 | 5.43 ± 0.07 |
| Novobiocin | 100 | 7.30 ± 0.05 | 4.67 ± 0.04 | 2.51 ± 0.01 | 3.65 ± 0.04 |
| Vismodegib | 50 | 4.09 ± 0.03 | 3.21 ± 0.01 | 2.01 ± 0.02 | 3.47 ± 0.01 |
| Sulfasalazine | 100 | 1.05 ± 0.08 | 1.03 ± 0.03 | 1.07 ± 0.01 | 1.41 ± 0.05 |
↵a The fold value is defined as the fluorescence of Pp-18 in cells in the presence of an inhibitor divided by the accumulation of Pp-18 in the absence of inhibitor. Data represent means ± S.D. of three observations.