TABLE 1
recUGT protein concentrations determined in BD Supersomes recombinant samples analyzed in duplicate
One optimal peptide was used for each UGT. The maximum and minimum concentration samples are circled and demonstrate the wide range of concentrations obtained. Lot numbers are shown in the sample names.
| pmol/mg Protein | pmol/mg Protein | ||||
|---|---|---|---|---|---|
| recUGT | Conc. | Mean ± S.D. (%CV) | recUGT | Conc. | Mean ± S.D. (%CV) |
| rUGT1A1-1 | 1359.0 | 1209.8 ± 249.1 (20.6) | rUGT1A9-1 | 441.1 | 490.5 ± 77.7 (15.8) |
| rUGT1A1-10 |  | rUGT1A9-8 | 604.3 | ||
| rUGT1A1-11 | 914.6 | rUGT1A9-9 | 476.4 | ||
| rUGT1A1-85244 | 1101.2 | rUGT1A9-06831 | 440.2 | ||
| rUGT1A3-1 | 536.2 | 473.2 ± 75.9 (16.0) | rUGT1A10-80018 | 709.5 | 738.5 ± 45.8 (6.2) |
| rUGT1A3-9 | 366.7 | rUGT1A10-19244 | 791.3 | ||
| rUGT1A3-10 | 421.3 | rUGT1A10-59891 | 714.7 | ||
| rUGT1A3-11 | 504.9 | ||||
| rUGT1A3-12 | 536.8 | rUGT2B4-1 | 354.4 | 355.3 ± 25.6 (20.6) | |
| rUGT2B4-2 | 367.6 | ||||
| rUGT1A4-9 | 537.5 | 417.3 ± 127.5 (30.6) | rUGT2B4-3 | 313.6 | |
| rUGT1A4-06150 | 303.4 | rUGT2B4-10714 | 381.9 | ||
| rUGT1A4-95375 | 517.3 | rUGT2B4-33191 | 358.9 | ||
| rUGT1A4-21161 | 310.8 | ||||
| rUGT2B7-1 | 797.7 | 914.7 ± 125.8 (13.8) | |||
| rUGT1A6-1 | 911.8 | 839.5 ± 172.8 (20.6) | rUGT2B7-9 | 918.5 | |
| rUGT1A6-7 | 943.6 | rUGT2B7-10 | 1148.7 | ||
| rUGT1A6-8 | 921.6 | rUGT2B7-11 | 926.8 | ||
| rUGT1A6-04294 | 581.1 | rUGT2B7-12 | 819.4 | ||
| rUGT2B7-95853 | 877.0 | ||||
| rUGT1A7-1 | 865.0 | 706.9 ± 176.2 (24.9) | |||
| rUGT1A7-13906 | 824.7 | rUGT2B15-6 | 220.4 | 243.8 ± 43.1 (17.7) | |
| rUGT1A7-10729 | 659.1 | rUGT2B15-7 | 236.8 | ||
| rUGT1A7-68106 | 478.9 | rUGT2B15-26316 | 306.5 | ||
| rUGT2B15-36575 |  | ||||
| rUGT1A8-7 | 697.8 | 598.8 ± 96.6 (16.1) | |||
| rUGT1A8-05599 | 504.8 | rUGT2B17-1 | 687.0 | 708.5 ± 28.0 (4.0) | |
| rUGT1A8-21754 | 593.7 | rUGT2B17-2 | 740.2 | ||
| rUGT2B17-3 | 698.2 | ||||