Test compound optimized settings for LC-MS/MS analysis.
| Compound | Transition | Ion Source Mode | Cone Voltage | Collision Energy |
|---|---|---|---|---|
| m/z | V | eV | ||
| Loperamide | 477.15 → 266.05 | Positive | 19.00 | 22.00 |
| Desmethyl-loperamide | 463.07 → 252.12 | Positive | 30.00 | 28.00 |
| Midazolam | 326.01 → 291.17 | Positive | 46.00 | 22.00 |
| Hydroxy-midazolama | 341.98 → 168.03 | Positive | 37.00 | 34.00 |
| Quinidine | 325.05 → 81.05 | Positive | 30.00 | 34.00 |
| Hydroxy-quinidine | 341.10 → 184.19 | Positive | 30.00 | 34.00 |
| Bupivacaine | 289.16 → 140.19 | Positive | 37.00 | 16.00 |
| Warfarin | 308.90 → 163.01 | Positive | 18.00 | 16.00 |
↵a Both 1-OH and 4-OH hydroxymidazolam were detected.