TABLE 1

Primers used in this study for cloning, sequencing, genotyping, and mutageneis (5′–3′)

Primer nucleotides are numbered relative to the UGT2B7 translation start site with the “A” of the initiator condon (ATG) positioned as +1. Incorporated restriction sites of both Mull (ACGCGT) and XhoI (CTCGAG) are underlined.

Cloning
2B7-1046FATAGACGCGTGACCTGAAGGAATACCTCAA
2B7-2076FATGTACGCGTGCCGGTTTCTAAGGCATATA
2B7-3026FATAGACGCGTTCAGCCTCCCACTTTGCAAT
2B7-4026FATCAACGCGTAGTGATGCTTCAGGAAACTT
2B7-4925FTACTACGCGTGAGCCCTGTAAACTCTTAAT
2B7-1RATATCTCGAGCCTGGTGCAATGCAAT
Sequencing
2B7-917RGTTATTTGGCTGTTGTCCTA
2B7-1947RTCATTAGCACTTTTTAGCAA
2B7-2907RGCCACTCAGGCACGTGTAGA
2B7-3867RCTGGATACAAAAGGTAAAGA
Genotyping
2B7-2579FGCATATTACCTACTTCCGGTC
2B7-1862RCCACCACCAAAATGAGTTAGCAA
Mutagenesis
2B7-900 A to G MTFGTGAGGAAGTGAGTCGGAGAACAAGCTAACC
2B7-900 A to G MTRGGTTAGCTTGTTCTCCGACTCACTTCCTCAC