TABLE 7

Mass spectral analysis of canagliflozin metabolites in mice, rats, dogs, and humans

Metabolite	[M + H]⁺	Diagnostic Product Ions	Biotransformation Pathways	Species
Canagliflozin	462	445, 427, 409, 391, 373, 349^a, 325^a, 267^a, 191^a, 147^a	—	—
M1	654	619, 601, 583, 547, 478^a, 443^a, 425, 407, 389, 347^a, 323^a, 191^a	Oxidation, glucuronidation	Mouse, rat
M2	654	619, 601, 583, 547, 478^a, 443^a, 425, 407, 389, 347^a, 323^a, 191^a	Oxidation, glucuronidation	Mouse
M4	494	459, 441, 423, 405, 387, 369, 339^a, 321^a, 191^a	Oxidation	Mouse, dog
M5	638	603, 585, 567, 549, 531, 507, 487, 462, 445^a, 427, 409, 391, 373, 349^a, 325, 267, 191^a	Glucuronidation	Mouse, rat, dog, human
M6	476	459, 441, 423, 405, 387, 325^a, 281, 263^a, 191^a, 147^a	Alcohol oxidation	Mouse, rat
M7	638	603, 585, 567, 549, 531, 507, 487, 462, 445^a, 427, 409, 391, 373, 349^a, 325, 267, 191^a	Glucuronidation	Mouse, rat, dog, human
M8	478	460, 443, 425, 407, 389, 347^a, 323^a, 191^a	Hydroxylation	Mouse, rat, dog
M9	478	461, 443, 425, 407, 389, 365^a, 341^a, 267, 207^a	Hydroxylation	Mouse, rat, dog, human
M10	478	460, 443, 425, 407, 389, 347, 283^a, 191^a	Oxidation	Rat
M12	492	475, 457, 439, 421, 403, 379, 355, 337, 323	Oxidation	Rat
M14	654	619, 601, 583, 565, 547, 523, 478, 461, 443, 425, 407, 389, 365, 341, 231, 207	Oxidation, glucuronidation	Rat
M15	654	601, 583, 565, 547, 523, 461, 425, 407, 389, 383, 365, 341, 267, 231, 207	Oxidation, glucuronidation	Rat
M16	654	461, 443, 425, 407, 376, 365, 341, 207	Oxidation, glucuronidation	Rat
M17	638	549, 531, 462, 427, 409, 391, 373, 349, 325, 267, 231, 191	Glucuronidation	Rat
M18	478	461, 443, 425, 407, 389, 383, 365, 341, 267, 249, 231, 207	Oxidation	Rat
M19	478	461, 443, 425, 407, 365, 353, 341, 207	Oxidation	Rat

↵^a Diagnostic fragments.