TABLE 1

Protein expression levels of metabolizing enzymes in microsomal fraction of humanized liver of PXB mice

Unit of protein expression is pmol/mg protein of microsomal fraction.

DonorALL (n =11) BD85 (n = 5) BD72 (n = 3)BD87 (n = 3)
MeanS.D.%CVnMeanS.D.% CVnMeanS.D.%CVnMeanS.D.%CVn
CYP2E151.89.718.71151.79.418552.97.815350.915.2303
CYP3A427.812.444.51126.711.141524.810.743332.418.9583
CYP2C9*21.714.265.5119.711.0010528.610.637334.814.4413
CYP1A220.76.430.91123.35.122516.57.143320.47.7383
CYP2C8*15.86.339.81110.21.818516.91.911324.12.193
CYP2A6*10.27.876.1112.930.4014512.41.613320.23.3163
CYP2C19*8.846.2570.8114.901.1524516.67.24337.592.99393
CYP2D6*7.072.9441.696.771.642451.2319.511.75183
CYP3A76.323.3152.356.323.31525LLOQLLOQ
CYP3A55.564.0973.566.563.66565LLOQ0.5791
CYP4A115.041.9739.194.851.994152.2916.261.24203
CYP2B6*1.900.9147.6111.680.493051.080.272533.110.45153
P450R*31.618.759.31112.41.714543.74.611351.21.733
UGT2B755.020.837.86N.D.43.011.426367.022.7343
UGT1A119.02.412.56N.D.19.73.518318.40.533
Na+/K+ ATPase10.41.514.5119.942.1422510.50.44311.10.983
γ-GTP2.640.3714.1112.570.331352.640.552132.740.37133
  • n, Number of determined samples; LLOQ, lower than the limit of quantification; N.D., not determined.

  • * Significant difference (P < 0.05) in protein expression levels among donors (BD85, BD72, and BD87).