Numerical Method, EII–Partial-Inactivation Model | Replot Method (All Data) | Replot Method (Linear Region) | ||||
---|---|---|---|---|---|---|
Repeat 1 | Repeat 2 | Repeat 1 | Repeat 2 | Repeat 1 | Repeat 2 | |
KI (μM) | 2.1 ± 0.29 | 2.4 ± 0.33 | 19.5 ± 7.2 | 24.2 ± 10.2 | ||
kinact (min−1) | 0.039 ± 0.001 | 0.030 ± 0.001 | 0.10 ± 0.01 | 0.13 ± 0.02 | ||
kinact/KI (nM−1 min−1) | 0.019 ± 0.003 | 0.013 ± 0.002 | 0.0051 ± 0.0020 | 0.0054 ± 0.0024 | ||
KI1 (μM) | 4.25 ± 1.0 | 3.81 ± 0.77 | ||||
KI2 (μM) | 20.36 ± 11.7 | 22.31 ± 10.05 | ||||
kinact1 (min−1) | 0.07 ± 0.02 | 0.07 ± 0.02 | ||||
kinact2 (min−1) | 0.19 ± 0.04 | 0.24 ± 0.04 | ||||
kinact1/KI1 (nM−1 min−1) | 0.017 ± 0.006 | 0.018 ± 0.006 | ||||
kinact2/KI2 (nM−1 min−1) | 0.0093 ± 0.0057 | 0.011 ± 0.005 | ||||
kprod (min−1) | 18.4 ± 0.17 | 20.5 ± 0.18 | ||||
kpartial (min−1) | 7.24 ± 0.35 | 6.59 ± 0.31 | ||||
kloss (min−1) | 0.005 ± 0.0009 | 0.0101 ± 0.0009 |