TABLE 5

Average parameter estimates (n = 100 repeats) for time-dependent inhibition with non-Michaelis-Menten (enzyme inhibitor inhibitor) inhibition of inactivation (II) kinetics

Data were simulated with the following parameters: II: K_I1 = 10 µM, K_I2 = 100 µM, k_inact1 = 0.025 min⁻¹, k_inact2 = 0.0025 min⁻¹, k_inact2/K_I2 = 2.5 × 10⁻⁵ min⁻¹ µM⁻¹.

Model (% Error in Simulated Data)	Parameter	Numerical Method^{^a}	Modified (II) Replot Method^{^a}	Standard Replot Method^{^a}^,^{^b}
II (10%)	K_I1	8.9–10.9	0.4–19.6	0.7–8.9
	k_inact1	0.021–0.031	0.012–0.032	0.011–0.022
	k_inact2	4 × 10⁻¹⁰–6 × 10⁻⁴	NE	NE
	k_inact2/K_I2 (×10⁻⁴)	1.8–2.6	0.0008–1.0	NE
II (5%)	K_I1	9.3–10.4	5.1–16.5	2.7–6.8
	k_inact1	0.022–0.027	0.018–0.031	0.013–0.019
	k_inact2	10⁻⁷–0.04	NE	NE
	k_inact2/K_I2 (×10⁻⁴)	1.9–2.3	0.0007–0.82	NE
II (2.5%)	K_I1	9.8–10.3	6.2–13.8	3.4–5.4
	k_inact1	0.024–0.026	0.021–0.029	0.015–0.018
	k_inact2	3 × 10⁻⁵–0.026	NE	NE
	k_inact2/K_I2 (×10⁻⁴)	2.0–2.2	0.003–1.0	NE

NE, not estimated.
↵^a Data are represented as a range of ±1 S.D. determined from the log normal distribution of 100 runs.
↵^b Parameters for the standard replot method are K_I and k_inact.