TABLE 1

MRM parameters for the major metabolites of six UGT probe substrates

UGT IsoformMetaboliteTransition PolarityCE
m/zeV
UGT1A1β-Estradiol-3-glucuronide447.0 → 271.0ES50
UGT1A3Chenodeoxycholic acid glucuronide567.5 → 391.5ES50
UGT1A4Trifluoperazine glucuronide584.5 → 408.5ES+35
UGT1A64-Hydroxyindole glucuronide310.0 → 134.0ES+20
UGT1A9Propofol glucuronide353.0 → 177.0ES35
UGT2B7Naloxone-3-glucuronide504.0 → 310.0ES+30
Internal standardEstrone glucuronide445.0 → 269.0ES38
  • CE, collision energy; ES, electrospray negative ion mode; ES+, electrospray positive ion mode.