TABLE 3

Parameter values for sodium fluorescein uptake kinetics in suspended rat hepatocytes at different ages

Values are the mean (±S.D.) of three different batches of hepatocyte preparation (except two batches at 6 weeks). Statistical significance is indicated by *P < 0.05, **P < 0.01, and ***P < 0.001 analysis of variance (and Dunnett's post hoc), compared with values at 8 weeks old (adult rat) as control condition.

Rat Age
<1 Week1 Week2 Weeks3 Weeks4 Weeks6 Weeks8 Weeks
Km (μM)55.9 ± 39.642.4 ± 17.356.8 ± 12.246.6 ± 1.544.6 ± 24.948.5 ± 8.438.2 ± 13.6
Vmax [pmol/(min × million cells)]67.0 ± 18.2**48.1 ± 6.8***59.4 ± 40.1***25.2 ± 11.2***51.2 ± 24.1***196.2 ± 7.8246.5 ± 101.1
CL (Vmax/Km) [μl/(min × million cells)]1.5 ± 0.7***1.3 ± 0.5***1.2 ± 1.0***0.5 ± 0.2***1.2 ± 0.2***4.1 ± 0.9**6.4 ± 0.6
Kd [μl/(min × million cells)]0.3 ± 0.2***0.3 ± 0.1***0.3 ± 0.1***0.3 ± 0.1***1.0 ± 0.5*1.4 ± 0.11.6 ± 0.2