TABLE 3

Suppression of paracetamol (CYP1A2), hydroxybupropion (CYP2B6), and 1′ hydroxymidazolam (CYP3A4) formation after treating HepaRG cells with increasing concentrations of LPS (1.37–333 ng/ml), IL-6 (0.123–30 ng/ml), and IL-18 (2.06–500 ng/ml)

Data were normalized to untreated cells and fit to a four-parameter dose-response model. Mean and standard deviation values are from three different plates. For L-18, no E_min is quoted owing to the lack of any dose-response.

Enzyme	Metabolite	LPS		IL6		IL18 EC₅₀
Enzyme	Metabolite	E_min	EC₅₀	E_min	EC₅₀	IL18 EC₅₀
		% of control	ng/ml	% of control	ng/ml
CYP1A2	Paracetamol	47.6 ± 7.2	>333	35.2 ± 7.7	8.96	>500
CYP2B6	Hydroxybupropion	47.4 ± 2.18	116	20.4 ± 1.28	0.970	>500
CYP3A4	1′-Hydroxymidazolam	39.1 ± 4.63	7.85	41.1 ± 3.44	2.89	>500