Glucuronidation assay conditions
| Substrate, Concentration | DMSO (%) | Incubation Time | Stopping Reagent |
|---|---|---|---|
| µM | min | µl | |
| 4-MU (200) | — | 5–30 | 4M PCA (10) |
| 17-α-Estradiol (50) | 1 | 5–60 | 5% Acetic acid in methanol (100) |
| Estradiol (50) | 1 | 5–60 | 5% Acetic acid in methanol (100) |
| Ethinylestradiol (50) | 5 | 10–60 | 5% Acetic acid in methanol (100), 1:5 4 M PCA:methanol (60) |
| Testosterone (50) | 5 | 10–60 | 4 M PCA (10) |
| Epitestosterone (50) | 5 | 30–60 | 1:5 4 M PCA:methanol (60), 4 M PCA (10) |
| Bilirubin (50) | 10 | 15–60 | Methanol (100) |
| Entacapone (200) | — | 5–60 | 1:5 4 M PCA:methanol (60), 4 M PCA (10) |
| Indomethacin (100) | 5 | 10–60 | Methanol (100) |
| Diclofenac (100) | — | 15–60 | Methanol (100) |
| Dexmedetomidine (1000) | — | 60–120 | 5% Acetic acid in methanol (100) |
| Levomedetomidine (500) | — | 60–120 | 5% Acetic acid in methanol (100) |
| S-propranolol (500) | — | 30–60 | Methanol (100) |
| R-propranolol (500) | — | 30–60 | Methanol (100) |