Oligonucleotides for the dUGT1As cloning
Inserted restriction site sequences are underlined.
| No. | Name | DNA sequence (5′->3′) |
|---|---|---|
| 1 | d1A-Eco-F | ttgaattcgaagcctatgttaatgcttctg |
| 2 | d1A-Sal-R | tttgtcgacgtgtgccttggatttgtgag |
| 3 | d1A1-Bam-F | aaggatccgtagcaaagacaagggcacca |
| 4 | d1A1-Eco-R | tcgaattccccggggatcttggatag |
| 5 | d1A2-Eco-R2 | ttcgaattccttggatagtgggtttttgctg |
| 6 | d1A2/3/4-Bam-F | aaggatccggtgctttgggcttctacag |
| 7 | d1A2/3/4-Eco-R | tcgaattcctgagacaatggcttc |
| 8 | d1A2/3/4-Nde-F | agaccatatgacgttcctgcaaagggtca |
| 9 | d1A2/3/4-Nde-R | acgtcatatggtctgaattccttgttaatctg |
| 10 | d1A6-Bam-F | gcggatccctgtcctctcccccagatg |
| 11 | d1A6-Eco-R | tcgaattcctgaggcaggactcccttca |
| 12 | d1A7/11-Bgl-F | caaagatctggattgccctggactgcag |
| 13 | d1A8/9/10-Bgl-F | caaagatctggattgccctgggctgcag |
| 14 | d1A7-Eco-R2 | ccgaattcctttggcaatgtctttcc |
| 15 | d1A8/9/10/11-Eco-R | ccgaattcctttggcaatggctttcc |