Kinetic parameters of DPP-4 activity using Gly-Pro-AMC
| Enzyme Source | Km | Vmax |
|---|---|---|
| µM | nmol/min/mg protein | |
| Human liver S9 fraction | 26.2 ± 2.48 | 2.70 ± 0.16 |
| Human liver cytosol | 21.3 ± 1.53 | 1.71 ± 0.06 |
| Human liver microsomes | 24.6 ± 1.11 | 5.06 ± 0.18 |
| Wild-type hDPP-4 | 54.0 ± 2.59 | 281 ± 11.0 |
| hR623Q | 45.6 ± 8.03 | 251 ± 12.6 |