TABLE 1

Multiple Reaction Monitoring parameters of peptides (calibrator and internal standard) selected for targeted analysis of hepatobiliary transporters in human (h), dog (d), monkey (m), and rat (r)

The labeled amino acid residue of the internal standard is shown in bold and italic.

Transporter	Signature Peptides	Parent Ion (z =2 )	Product Ions (z = 1)		Cone	CE	RT	On-column Calibration Range^a
			1	2
					V		min	fmol
dBcrp	TIIFSIHQPR	606.4	997.1	884.1	23	23	9.6	0.16–5.2
dBcrp	TIIFSIHQPR	611.4	747.4	894.4	23	23	9.6	0.16–5.2
dBcrp,mBcrp, and rBcrp	ENLQFSAALR	575.5	665.0	793.0	15	15	9.9	0.19–7.5
dBcrp,mBcrp, and rBcrp	ENLQFSAALR	579.8	674.4	802.4	15	15	9.9	0.19–7.5
dBsep, mBsep, rBsep, and hBSEP	STALQLIQR	515.2	529.1	657.1	18	18	9.6	0.06–6.3
dBsep, mBsep, rBsep, and hBSEP	STALQLIQR	520.3	539.4	667.4	18	18	9.6	0.06–6.3
dBsep, mBsep, rBsep, and hBSEP	STSIQLLER	523.9	530.3	658.3	17	17	9.5	0.07–6.9
dBsep, mBsep, rBsep, and hBSEP	STSIQLLER	528.8	540.3	668.4	17	17	9.5	0.07–6.9
rMate1	LLLSGFQEELR	652.8	965.1	1078.1	18	18	11.3	0.12–12
rMate1	LLLSGFQEELR	657.9	975.5	1088.6	18	18	11.3	0.12–12
hMATE1 and mMate1	HVGVILQR	461.2	529.2	685.2	15	15	7.5	0.13–13
hMATE1 and mMate1	HVGVILQR	466.3	539.4	695.4	15	15	7.5	0.13–13
hMATE1	GGPEATLEVR	514.7	816.9	688.0	16	16	8.0	0.11–7.5
hMATE1	GGPEATLEVR	519.8	627.4	698.4	16	16	8.0	0.11–7.5
dMdr1, mMdr1, and rMdr1	NTTGALTTR	467.8	490.2	719.1	16	16	6.4	0.10–6.4
dMdr1, mMdr1, and rMdr1	NTTGALTTR	472.8	628.4	729.4	16	16	6.4	0.10–6.4
dMdr1, mMdr1, and rMdr1	LANDAAQVK	465.2	745.0	816.0	15	15	6.1	0.11–7.3
dMdr1, mMdr1, and rMdr1	LANDAAQVK	469.3	753.4	824.4	15	15	6.1	0.11–7.3
dMrp2, mMrp2, and rMrp2	LTIIPQDPILFSGSLR	885.4	989.1	1329.0	26	26	13.8	0.05–25
dMrp2, mMrp2, and rMrp2	LTIIPQDPILFSGSLR	890.5	999.6	1339.7	26	26	13.8	0.05–25
rMrp2	FFDTTPTGR	521.2	632.0	894.0	14	14	8.5	0.24–16
rMrp2	FFDTTPTGR	526.3	642.3	904.4	14	14	8.5	0.24–16
dMrp3, mMrp3, and rMrp3	FYVATSR	422.2	434.1	533.1	11	11	7.7	0.15–15
dMrp3, mMrp3, and rMrp3	FYVATSR	427.2	706.4	543.3	11	11	7.7	0.15–15
dMrp3	LSVFWDYAK	564.4	928.0	682.1	10	10	11.8	0.13–12.8
dMrp3	LSVFWDYAK	568.8	936.5	690.3	10	10	11.8	0.13–12.8
hMRP3	ADGALTQEEK	531.2	634.0	747.0	13	13	6.2	0.13–8.5
hMRP3	ADGALTQEEK	535.3	642.3	755.4	13	13	6.2	0.13–8.5
rMrp3	LLEAWQK	444.7	461.1	661.1	12	12	8.9	0.23–14.6
rMrp3	LLEAWQK	448.3	654.4	669.3	12	12	8.9	0.23–14.6
dMrp4 and rMrp4	GTYTEFLK	479.3	536.1	800.0	10	10	9.6	0.22–13.2
dMrp4 and rMrp4	GTYTEFLK	483.8	544.3	808.4	10	10	9.6	0.22–13.2
hMRP4	AEAAALTETAK	538.2	733.0	874.9	13	13	7.0	0.41–6.9
hMRP4	AEAAALTETAK	542.3	741.4	883.5	13	13	7.0	0.41–6.9
mMrp4	LNTIIDSDR	523.6	605.0	718.0	14	14	8.3	0.13–12.8
mMrp4	LNTIIDSDR	528.8	615.3	728.4	14	14	8.3	0.13–12.8
rMrp4	LNTIIDSDK	509.4	577.1	690.1	15	15	8.0	0.23–13.6
rMrp4	LNTIIDSDK	513.8	585.3	698.4	15	15	8.0	0.23–13.6
hNTCP, mNtcp, and rNtcp	GIYDGDLK	440.7	547.1	710.1	12	12	8.2	0.22–15
hNTCP, mNtcp, and rNtcp	GIYDGDLK	444.7	555.3	718.3	12	12	8.2	0.22–15
rNtcp	AAATEDATPAALEK	679.8	915.1	729.6	14	14	7.7	0.16–27
rNtcp	AAATEDATPAALEK	683.9	737.4	923.5	14	14	7.7	0.16–27
dOatp1a2	IYDSTNFR	508.2	739.0	902.0	12	12	7.9	0.19–12.1
dOatp1a2	IYDSTNFR	513.2	749.3	912.4	12	12	7.9	0.19–12.1
dOatp1b4	TSPEIVEK	451.7	357.6	488.2	14	14	6.8	0.07–18.1
dOatp1b4	TSPEIVEK	455.8	625.4	722.4	14	14	6.8	0.07–18.1
dOatp2b1	VLAASASTVSK	517.2	679.1	—	15	15	6.9	0.11–7.1
dOatp2b1	VLAASASTVSK	521.3	829.5	942.5	15	15	6.9	0.11–7.1
mOatp1b1	LNPIGIAK	413.3	501.0	598.1	18	18	8.5	0.07–16.2
mOatp1b1	LNPIGIAK	417.3	509.4	606.4	18	18	8.5	0.07–16.2
mOatp1b3	IVQPELK	413.8	486.2	614.1	18	18	7.6	0.07–18.0
mOatp1b3	IVQPELK	417.8	494.3	622.4	18	18	7.6	0.07–18.0
mOatp2b1	VLLQTLR	421.7	517.1	630.1	17	17	9.2	0.12–7.4
mOatp2b1	VLLQTLR	426.8	527.3	753.5	17	17	9.2	0.12–7.4
rOatp1a1	EENLGITK	452.2	418.1	645.1	13	13	7.4	0.23–14.7
rOatp1a1	EENLGITK	456.2	539.4	653.4	13	13	7.4	0.23–14.7
rOatp1a4	TFQFPGDIESSK	678.3	832.1	979.1	14	14	10.2	0.10–10
rOatp1a4	TFQFPGDIESSK	682.3	840.4	987.5	14	14	10.2	0.10–10
rOatp1b2	SVQPELK	400.7	486.0	614.4	10	10	6.8	0.15–15
rOatp1b2	SVQPELK	404.7	494.3	622.4	10	10	6.8	0.15–15
dOct1	LSPSFVDLFR	590.9	490.8	883.1	22	22	13.5	0.09–5.9
dOct1	LSPSFVDLFR	595.8	893.5	990.5	22	22	13.5	0.09–5.9
hOCT1 and mOct1	LSPSFADLFR	576.7	768.0	855.0	23	23	12.0	0.09–30
hOCT1 and mOct1	LSPSFADLFR	581.8	778.4	865.4	23	23	12.0	0.09–30
rOct1	GVALPETIEEAENLGR	849.3	917.1	1357.1	30	30	11.6	0.12–8.0
rOct1	GVALPETIEEAENLGR	854.4	927.4	1367.7	30	30	11.6	0.12–8.0

↵^a Low end of this range reflects the lower limit of quantification of the assay.
CE, collision energy; RT, retention time.