TABLE 1

Enzyme activities of main DMEs

Mice were treated with silybin (50 mg/kg or 150 mg/kg, once daily) for a consecutive 2 weeks. Twenty-four hours after the last administration, the mice were sacrificed and their livers were immediately removed to prepared microsomes. Enzyme activities of main P450s and UGTs were tested using the typical probe substrate approach. Data are shown as mean ± S.D. (n = 3).

Substrates	Activity of DMEs (pmol/min/mg microsome protein)
Substrates	Vehicle	Silybin (50 mg/kg)	Silybin (150 mg/kg)
Phenacetin	516.23 ± 79.93	440.59 ± 33.02	319.10 ± 27.71^{^a}
Diclofenac	159.06 ± 4.39	168.32 ± 8.92	126.97 ± 1.51^{^a}
Chlorzoxazone	1446.31 ± 29.98	1553.31 ± 59.82	1332.36 ± 81.52
Midazolam	1291.11 ± 98.69	1305.59 ± 29.69	907.76 ± 60.01^{^b}
β-estradiol	718.71 ± 42.12	621.71 ± 16.36^{^a}	419.42 ± 21.95^{^b}
4-MU	75,459.46 ± 9478.70	109,474.65 ± 2324.02	83,348.31 ± 8070.37
Naloxone	286.74 ± 38.05	295.84 ± 31.74	289.04 ± 29.78

↵^a P < 0.01 represents the comparison of activities of DMEs between Silybin treated groups and vehicle group.
↵^b P < 0.001.