Kinetics of esculetin, 4-methylesculetin, 4-phenylesculetin, and 4-hydroxymethyl esculetin in HLM and UGTs
Km and Vmax values are expressed as best fit values ± S.E.
| Compound | Km | Vmax | CLint | ||||||
|---|---|---|---|---|---|---|---|---|---|
| HLM | 1A6 | 1A9 | HLM | 1A6 | 1A9 | HLM | 1A6 | 1A9 | |
| μM | nmol/min/mg | μl/min/mg | |||||||
| Esculetin | 347.9 ± 19.0 | 178.1 ± 8.9 | 115.1 ± 10.3 | 11.6 ± 0.3 | 3.2 ± 0.1 | 2.0 ± 0.1 | 33.3 | 17.9 | 17.3 |
| 4-ME | 85.5 ± 3.9 | 69.1 ± 4.9 | 30.5 ± 1.8 | 33.4 ± 0.5 | 19.3 ± 0.4 | 4.7 ± 0.1 | 390.6 | 279.3 | 154.0 |
| 4-PE | 7.7 ± 0.5 | — | 3.5 ± 0.3 | 12.0 ± 0.2 | — | 5.2 ± 0.1 | 1558.4 | — | 1485.7 |
| 4-HME | 748.8 ± 177.0 | 789.9 ± 85.1 | — | 10.3 ± 1.3 | 5.3 ± 0.3 | — | 13.7 | 6.7 | — |