TABLE 1

Each numerical entry represents the relative abundance (percentage) of all GSH conjugates combined that were formed via conjugate addition during the reaction of the compound listed at left

The values were estimated based on the ratio of UV peak areas of the GSH conjugates detected following 30 minutes of incubation to that of the respective parent compound at 0 minute. The values represent the mean of the duplicate measurements for each of the incubations.

Liver Subcellular Fraction or BufferCombined Amount of GSH Conjugates [Percentage of the Original Parent Drug Level (10 µM)]
Potassium Phosphate BufferHeat-Inactivated Liver S9Liver MicrosomesLiver Cytosol
SpeciesNAHumanRatHumanHumanRatRatHumanRat
Cofactor(s)GSHGSHGSHGSH + NADPHGSHGSH + NADPHGSHGSHGSH
Compound 123344610548
Compound 211134891656
Compound 3MSaMSMSMS<1<1<1<17
Compounds 4–8NDNDNDNDNDNDNDNDND
  • NA, not applicable; ND, not detected.

  • a Detected only by MS with no UV response.