TABLE 1

Primers for rat liver CYP450 mRNA isolation and quantitative polymerase chain reaction

Gene (Rat)	Human Ortholog	Sequence	Annealing Temperature (°C)
Rat PXR	Nr1i2	Forward: 5′-GGCCGATGTGTCAACCTACA-3′	55
Rat PXR	Nr1i2	Reverse: 5′-AAGCCGCCATTAGGGTCTTC-3′	55
Rat CAR	Nr1i3	Forward: 5′-AGCAAGGCCCAGAGACGCCA-3′	60
Rat CAR	Nr1i3	Reverse: 5′-CCGGAGGCCTGAACTGCACAAA-3′	60
CYP1A2	CYP1A2	Forward: 5′-TACAACTCTGCCAGTCTCCAG-3′	55
CYP1A2	CYP1A2	Reverse: 5′-TTGGGAACCTGGGTCCTTGT-3′	55
CYP2C11	CYP2C9	Forward: 5′-AAGCACAATCCGCAGTCTGA-3′	55
CYP2C11	CYP2C9	Reverse: 5′-GGCATCTGGCTCCTGTCTTT-3′	55
CYP2D2	CYP2D6	Forward: 5′-TATTGAGTGTTGGCCAGTGGT-3′	60
CYP2D2	CYP2C9	Reverse: 5′-GTGGACACAGAGAATCGCCT-3′	60
CYP3A2	CYP3A4	Forward: 5′-CTAAAGGTTCTGCCACGGGA-3′	55
CYP3A2	CYP3A4	Reverse: 5′-CCATGCATCAAGAGCAGTCA-3′	55
Cyclophilin	—	Forward: 5′-ATGTGGTCTTTGGGAAGGTG-3′	55
Cyclophilin	—	Reverse: 5′-GAAGGAATGGTTTGATGGGT-3′	55