TABLE 1

Effects of IL-1β and IL-6 on metabolism enzymes mRNA expression in Hep:KC (1:0 and 1:0.4) cell cultures

Metabolism EnzymesExposure to IL-1β (6.25–5000 pg/ml), 4 DaysExposure to IL-6 (6.25–5000 pg/ml), 4 Days
EffectsaKC-Dependent
Effects?EffectsaKC-Dependent
Effects?
CYP1A1↔—↔—
CYP1A2↓Yes↓—
CYP2A6ND—ND—
CYP2B6↔—↓—
CYP2C8↓Yes↓—
CYP2C9↓Yes↓—
CYP2C9↔—↔—
CYP2C19ND—↔—
CYP2D6↔—↔—
CYP2E1↔—↔—
CYP3A4↓Yes↓—
GSTA1↓bYes↓—
GSTA2↓bYes↓—
SULT1A1ND—ND—
SULT1A2ND—ND—
UGT1A1↓Yes↓—
UGT2B7↓bYes↓—

  • ↵a Effects were noted as noneffect (↔) or downregulated (↓) ≤50% of untreated control in a concentration-dependent manner. Samples with Ct value >32 were designated as nondetectable (ND).

  • ↵b Effects were observed only in Hep:KC 1:0.4 but not 1:0 cultures.