TABLE 1

Effects of IL-1β and IL-6 on metabolism enzymes mRNA expression in Hep:KC (1:0 and 1:0.4) cell cultures

Metabolism EnzymesExposure to IL-1β (6.25–5000 pg/ml), 4 DaysExposure to IL-6 (6.25–5000 pg/ml), 4 Days
EffectsaKC-Dependent
Effects?EffectsaKC-Dependent
Effects?
CYP1A1
CYP1A2Yes
CYP2A6NDND
CYP2B6
CYP2C8Yes
CYP2C9Yes
CYP2C9
CYP2C19ND
CYP2D6
CYP2E1
CYP3A4Yes
GSTA1bYes
GSTA2bYes
SULT1A1NDND
SULT1A2NDND
UGT1A1Yes
UGT2B7bYes
  • a Effects were noted as noneffect (↔) or downregulated (↓) ≤50% of untreated control in a concentration-dependent manner. Samples with Ct value >32 were designated as nondetectable (ND).

  • b Effects were observed only in Hep:KC 1:0.4 but not 1:0 cultures.