Characteristics of the CYP2B6 variant constructs
| P450 contenta | Cytochrome c reductase activity | Cytochrome b5 content | P450/reductase/b5 ratio | |
|---|---|---|---|---|
| pmol/mg | μmol/min per milligram protein | nmol/mg protein | ||
| CYP2B6.1 + P450 reductase + cytochrome b5 | 77 | 4.5 | 0.25 | 1:18:3 |
| CYP2B6.4 + P450 reductase + cytochrome b5 | 107 | 3.3 | 0.28 | 1:10:3 |
| CYP2B6.5 + P450 reductase + cytochrome b5 | 172 | 4.1 | 0.49 | 1:7:3 |
| CYP2B6.6 + P450 reductase + cytochrome b5 | 458 | 4.0 | 0.28 | 1:2:1 |
| CYP2B6.9 + P450 reductase + cytochrome b5 | 198 | 5.9 | 0.32 | 1:9:2 |
| CYP2B6.18 + P450 reductase + cytochrome b5 | 0 | 2.1 | 0.34 | |
| CYP2B6.1 + P450 reductase | 64 | 3.6 | 1:18 | |
| CYP2B6.4 + P450 reductase | 28 | 2.6 | 1:30 | |
| CYP2B6.5 + P450 reductase | 29 | 2.6 | 1:28 | |
| CYP2B6.6 + P450 reductase | 37 | 2.9 | 1:24 | |
| CYP2B6.9 + P450 reductase | 28 | 3.0 | 1:34 | |
| CYP2B6.18 + P450 reductase | 0 | 1.9 |
↵a Determined spectrophotometrically from CO difference spectra, as described in Materials and Methods.