TABLE 1

Summary of peptide sequences and multiple reaction monitoring (MRM) transitions used to quantitate the four renal transporter proteins with LC-MS/MS

TransporterSequenceMRM Transition (m/z)a
Mass (Da)Q1Q3-1Q3-2Q3-3
OAT2VLLPLHFLLPIFLAAVPAHR2238747982835480
VLLPLHFLLPIFLAAVPAHbR2244749988841486
OCT2SLPASLQR871436672574503
SLPASLQRb881441682584513
MATE1GGPEATLEVR1027515688617458
GGPEATLEVRb1037520698627463
MATE2KYLQNQK792397630517389
YLQNQKb800401638525397

  • ↵a Theoretical m/z value of doubly charged ions of intact peptide (Q1) assumed as precursor ions; singly charged fragment ions derived from precursor ion indicated as Q3-1, Q3-2, and Q3-3.

  • ↵b Amino acid residues labeled with stable isotope (13C and 15N).