Summary of peptide sequences and multiple reaction monitoring (MRM) transitions used to quantitate the four renal transporter proteins with LC-MS/MS
Transporter | Sequence | MRM Transition (m/z)a | ||||
---|---|---|---|---|---|---|
Mass (Da) | Q1 | Q3-1 | Q3-2 | Q3-3 | ||
OAT2 | VLLPLHFLLPIFLAAVPAHR | 2238 | 747 | 982 | 835 | 480 |
VLLPLHFLLPIFLAAVPAHbR | 2244 | 749 | 988 | 841 | 486 | |
OCT2 | SLPASLQR | 871 | 436 | 672 | 574 | 503 |
SLPASLQRb | 881 | 441 | 682 | 584 | 513 | |
MATE1 | GGPEATLEVR | 1027 | 515 | 688 | 617 | 458 |
GGPEATLEVRb | 1037 | 520 | 698 | 627 | 463 | |
MATE2K | YLQNQK | 792 | 397 | 630 | 517 | 389 |
YLQNQKb | 800 | 401 | 638 | 525 | 397 |