TABLE 1

Sequence of primers used for RT-PCR and quantitative RT-PCR

IsoformsPrimersSequencePositionAnnealing TemperatureReferences
°C
UGT1A1UGT1A1-S5′-CCT TGC CTC AGA ATT CCT TC-3′696–71562Nakamura et al. (2008)
UGT1A-AS5′-ATT GAT CCC AAA GAG AAA ACC AC-3′907–929Nakamura et al. (2008)
UGT1A3UGT1A3-S5′-TGT TGA ACA ATA TGT CTT TGG TCT-3′347–37062Nakamura et al. (2008)
UGT1A3-AS5′-CAC AGG ACT GTC TGA GGG ATT TT-3′985–1007
UGT1A4UGT1A4-S5′-CCT GCT GTG TTT TTT TGG AGG T-3′502–52354Izukawa et al. (2009)
UGT1A-AS5′-ATT GAT CCC AAA GAG AAA ACC AC-3′907–929Nakamura et al. (2008)
UGT1A5UGT1A5-S5′-ACA ATA TGT CTT TGA TCA TA-3′353–37254Nakamura et al. (2008)
UGT1A5-AS5′-AGA AAC AGC ATG GCA AAG-3′667–684Nakamura et al. (2008)
UGT1A6UGT1A6-S5′-AGA GAA TTT CTG CAG GGG TTT T-3′26–4754Nakamura et al. (2008)
UGT1A6-AS5′-TTG GAT TCT TTC AAA AGC-3′195–212Nakamura et al. (2008)
UGT1A7UGT1A7-S5′-CCC CTA TTT TTT CAA AAA TGT CTT-3′660–68354Izukawa et al. (2009)
UGT1A-AS5′-ATT GAT CCC AAA GAG AAA ACC AC-3′907–929Nakamura et al. (2008)
UGT1A8UGT1A8-S5′-GGT CTT CGC CAG GGG AAT AG-3′498–51762Nakamura et al. (2008)
UGT1A-AS5′-ATT GAT CCC AAA GAG AAA ACC AC-3′907–929Nakamura et al. (2008)
UGT1A9UGT1A9-S5′-GAA CAT TTA TTA TGC CAC CG-3′646–66562Nakamura et al. (2008)
UGT1A-AS5′-ATT GAT CCC AAA GAG AAA ACC AC-3′907–929Nakamura et al. (2008)
UGT1A10UGT1A10-S5′-CTC TTT CCT ATG TCC CCA ATG A-3′557–57862Nakamura et al. (2008)
UGT1A-AS5′-ATT GAT CCC AAA GAG AAA ACC AC-3′907–929Nakamura et al. (2008)
Cyp2b10Cyp2b10-S5′-GCA AGC CAT GTT GCT CCT AA-3′1405–142460
Cyp2b10-AS5′-CTT GGA GCC CTG GAG ATT TGG-3′1604–1624
Cyp3a11Cyp3a11-S5′-CTC AAT GGT GTG TAT ATC CCC-3′1228–124860Fujiwara et al. (2012)
Cyp3a11-AS5′-CCG ATG TTC TTA GAC ACT GCC-3′1630–1650Fujiwara et al. (2012)
GAPDHGAPDH-S5′-CCA GGG CTG CTT TTA ACT C-3′56–7462Nakamura et al. (2008)
GAPDH-AS5′-GCT CCC CCC TGC AAA TGA-3′330–347Nakamura et al. (2008)
CPHCPH-S5′-CAG ACG CCA CTG TCG CTT T-3′5–2362Fujiwara et al. (2012)
CPH-AS5′-TGT CTT TGG AAC TTT GTC TGC AA-3′115–137Fujiwara et al. (2012)
  • GAPDH, glyceraldehyde 3-phosphate dehydrogenase.