Comparison between typical and screened reaction conditions to optimize cost, efficiency, and yield of milk thistle glucuronides
| Reagent | Typical Conditiona | Screened Condition(s) |
|---|---|---|
| Tris-HCl (pH 7.4) (mM)b | 100 | 100 |
| MgCl2 (mM)b | 5 | 5 |
| BSA (%, w/v)b | 0.5 | 0.5 |
| Alamethicin (μM)b | 25 | 25 |
| Silybin A (mM)b | 0.4 | 0.4 |
| UDPGA (mM) | 4.0 | 4.0, 0.8 |
| Enzyme source | HLMs | BLMs, bovine S9 fraction |
| Enzyme source concentration (mg/ml protein) | 1.00 | 1.00, 0.50, 0.25 (BLMs); 2.00, 1.00, 0.50 (S9) |