Reagent | Typical Conditiona | Screened Condition(s) |
---|---|---|
Tris-HCl (pH 7.4) (mM)b | 100 | 100 |
MgCl2 (mM)b | 5 | 5 |
BSA (%, w/v)b | 0.5 | 0.5 |
Alamethicin (μM)b | 25 | 25 |
Silybin A (mM)b | 0.4 | 0.4 |
UDPGA (mM) | 4.0 | 4.0, 0.8 |
Enzyme source | HLMs | BLMs, bovine S9 fraction |
Enzyme source concentration (mg/ml protein) | 1.00 | 1.00, 0.50, 0.25 (BLMs); 2.00, 1.00, 0.50 (S9) |