TABLE 2

Unbound fraction of TREO and S,S-EBDM in rat plasma and brain homogenate

Matrix	Analyte	Concentration^{^a}	Unbound fraction^{^b}
Plasma	TREO	2.3 μM	0.962 ± 0.137
		57 μM	0.940 ± 0.061
		2000 μM	0.960 ± 0.163
	S,S-EBDM	3.5 μM	1.083 ± 0.211
	S,S-EBDM	87 μM	0.986 ± 0.119
Brain homogenate	TREO	2.3 μM	1.063 ± 0.172
		5.7 μM	0.999 ± 0.081
		17.2 μM	1.000 ± 0.108
	S,S-EBDM	3.5 μM	1.092 ± 0.079
	S,S-EBDM	8.7 μM	0.974 ± 0.075

↵^a The used concentrations covered the range observed in the JR and the YAR that received TREO 500 mg/kg b.wt. as an intravenous bolus.
↵^b At each concentration level of TREO or S,S-EBDM the unbound fraction was determined using five lots of plasma and brain homogenate obtained from different 2-mo-old rat donors.