TABLE 2

Predicted drug clearance rates in MPCCs compared with conventional confluent monolayers created using the same donor (RTM)

MPCCs were stabilized for 7 days prior to dosing with drugs for up to 7 days, whereas conventional monolayers were stabilized for 1 day prior to dosing with drugs for up to 4 days. The dash indicates that the drug did not turn over sufficiently in the model system to predict a clearance rate.

In Vivo Observed Pharmacokinetic Parameter ValuesPredicted Clearance
fu = Reported Literature Valuesfu = 1
Compound NameIn Vivo ClearancefuMPCCsConventional MonolayerMPCCsConventional Monolayer
ml/min 
per kilogramml/min per kilogram
Verapamil19.50.106.790.817.365.98
Naloxone180.5414.4511.9816.8714.93
Timolol10.10.905.390.955.821.05
Methylprednisolone6.10.234.670.9111.643.46
Erythromycin5.60.100.710.735.445.59
Theophylline1.10.410.340.430.811.02
Lorazepam10.090.460.114.151.13
Diazepam0.530.020.210.036.31.27
Tolbutamide0.380.050.210.063.491.19
Naproxen0.190.010.189.75
Within 2-fold50%10%60%50%
Within 3-fold80%20%60%60%
Within 4-fold90%20%60%80%