TABLE 3

Individual abundances of human intestinal and Caco-2 cell monolayer, with REFs for Na/K-ATPase, P-gp, and BCRP

Standard deviations are given in parentheses. *Denotes mean relative error when accounting for individual samples.

Sample	Na/K-ATPase Abundance (fmol Transporter/μg Total Membrane Protein)			P-gp Abundance (fmol Transporter/μg Total Membrane Protein)			BCRP Abundance (fmol Transporter/μg Total Membrane Protein)
Sample	Bertin Pharma	University  of Manchester	Relative  Error (%)	Bertin Pharma	University  of Manchester	Relative  Error (%)	Bertin Pharma	University  of Manchester	Relative Error (%)
Distal jejunum 1	43.39	41.93	−3	1.10	3.07	64	3.19	2.28	−29
Distal jejunum 2	37.48	34.58	−8	0.40	0.98	59	0.98	1.92	49
Distal jejunum 3	37.11	29.58	−25	0.80	1.62	51	2.01	3.49	42
Distal ileum	50.72	24.47	−107	BLQ	0.20	—	0.44	1.6	73
Caco-2 21d (# 1)	51.56	82.47	37	1.89	5.21	64	1.7	1.19	−43
Caco-2 21d (# 2)	56.75	47.16	−20	2.27	4.5	50	1.94	1.16	−67
Caco-2 21d (# 3)	65.86	56.75	−16	2.07	4.31	52	1.94	1.12	−73
Caco-2 29d (# 1)	49.3	77.43	36	1.64	8.27	80	1.34	1.12	−20
Caco-2 29d (# 2)	74.81	54.32	−38	2.29	6.78	66	1.64	0.72	−128
Caco-2 29d (# 3)	52.45	58.83	11	1.98	6.78	71	1.51	0.75	−101
Caco-2 21d HP	90.43	52.33	−73	7.84	5.71	37	2.73	1.64	−66
Mean distal jejunum	39.3 (±3.5)	35.4 (±6.2)	–12*	0.8 (±0.3)	1.9 (±1.1)	58*	2.1 (±1.1)	2.6 (±0.8)	17*
Mean Caco-2 21d	58.1 (±7.2)	62.1 (±18.3)	–0.4*	2.1 (±0.2)	4.7 (±0.5)	55*	1.9 (±0.1)	1.2 (±0.0)	–62*
Mean Caco-2 29d	58.9 (±14)	63.5 (±12)	3 *	2.0 (±0.3)	6.9 (±1.3)	71*	1.3 (±0.3)	0.9 (±0.2)	–83*
Overall mean	55.4 (±16.1)	50.9 (±18.1)	–19*	2.0 (±2.1)	4.8 (±3.5)	63*	1.8 (±0.8)	1.5 (±0.9)	–33*
REF (jejunum: Caco-2-21d)	0.68	0.58	—	0.37	0.4	—	1.11	2.22	—