TABLE 4 

Activity-based rat scaling factors for MPPMC (mg of protein per million cells), MPPGL (mg of protein/g of liver) and HPGL (million cells/g of liver)

Scaling factors were calculated for atazanavir following the described methodology and compared with scaling factors derived from verapamil data and “conventional” rat scaling factors as reported in literature. Values are means ± S.D.

Scaling factorActivity-BasedP450-Content Based
AtazanaviraVerapamilb
Fresh SRHCryo SRHFresh SRHCryo SRHConventional (rat)
MPPMC0.27 ± 0.10.24 ± 0.10.30 ± 0.020.28 ± 0.030.37c
MPPGL40 ± 1646 ± 2380 ± 1543 ± 1444.5b/45d/61e
HPGL150 ± 31191 ± 49269 ± 44153 ± 27120d/163e
  • a Determined in the present study using atazanavir.

  • b Determined in our previous study using verapamil (Nicolaï et al., 2015).

  • c Calculated from the conventional MPPGL and HPGL stated by Smith et al. (2008).

  • d Reported by Barter et al. (2007).

  • e Reported for the Wistar rat by Smith et al. (2008).