Transporter abundance levels in the HEK293 cells (SLC transporters), inverted membrane vesicles (ABC transporters), and sandwich-cultured human hepatocytes (SCHHs)

Transporter expression was determined from abundance levels of surrogate peptides measured by targeted LC-MS/MS analysis (CV of the quantification methods <15%) (Balogh et al., 2012; Li et al., 2008b; Li et al., 2009; Qiu et al., 2013).

Surrogate peptide expression levels (pmol/mg membrane protein)
TransporterHEK293 CellsMembrane VesiclesSCHH UU113SCHH UU115SCHH UU112SCHH UU125
NTCP (SLC10A1)19.7NA0.380.530.630.27
OATP1B1 (SLCO1B1)12.1aNA3.824.462.054.64
OATP1B3 (SLCO1B3)4.71aNA0.451.010.791.69
OATP2B1 (SLCO2B1)57.3aNA1.301.661.670.65
BCRP (ABCG2)NA2600.220.390.330.11
MDR1 (ABCB1)NA2800.951.190.830.95
MRP3 (ABCC3)NA40.00.970.961.601.64
  • NA, not applicable.

  • a Values reprinted with permission from Karlgren et al. (2012b).