Metabolite/Component | Sample Collection Time | Exposure | |||||
---|---|---|---|---|---|---|---|
1 Hour | 24 Hours | 96 Hours | 168 Hours | AUC0–168h | Percentage of AUC0–168h | AUCinf | |
nM | nM | nM | nM | nM·h | % | nM·h | |
P1 (front peak) | 5.81 | 2.47 | 0.754 | 0.215 | 249 | 0.395 | 262 |
P1.8 (front peak) | 0.700 | 0.408 | 0.158 | 0.0321 | 40.3 | 0.0640 | 42.1 |
M7 (cysteine conjugate) | 1.58 | 0.515 | 0.166 | 0.0463 | 57.1 | 0.0907 | 59.9 |
M2a (LYS-MCC-DM1) | ND | 0.408 | 0.232 | 0.0676 | 38.5 | 0.0611 | 42.5 |
M6 (Maysine) | 1.06 | 0.419 | 0.116 | 0.0248 | 41.9 | 0.0664 | 43.1 |
M1a (MCC-DM1) | 1.72 | 0.327 | ND | ND | 36.2 | 0.0575 | |
M5 (derivatized DM1)b | 16.3 | 2.93 | 0.821 | 0.189 | 400 | 0.635 | 410 |
Sum of additional metabolitesc | 2.23 | 0.199 | 0.230 | 0.0895 | 56.0 | 0.0888 | 60.6 |
Total components detected | 13.1 | 4.74 | 1.66 | 0.475 | 519 | 0.824 | 549 |
Lost during sample processingd | 1443 | 588 | 205 | 67.4 | 62,469 | 99.2 | NC |
Total components in original sample | 1456 | 593 | 207 | 67.9 | 62,988 | 100 | 67,500 |
NC, not calculated; ND, not detected.
↵a Peaks were identified by retention time only.
↵b DM1-NEM measured after TCEP treatment is shown in italic (corresponds to possible disulfide-bonded DM1 with endogenous components in serum).
↵c Corresponds to the sum of the residual RA measured in each radiochromatogram.
↵d Including ADCs and/or aggregates/complexes.