Primers used for targeted bisulfite sequencing
| Region | Primer Name | Sequence | Product Size |
|---|---|---|---|
| bp | |||
| CYP3A4 proximal | CYP3A4-842 for | attagaaagttagaagggatgatatgtaga | 650 |
| CYP3A4-193 rev | tacaattaaaaaaaacttctccaccttaaa | ||
| CYP3A4-748 for (nest) | ttttggtagggattagagttatgatagg | 459 | |
| CYP3A4-256 rev (nest) | aacctcctttaaattcatattctataaaat | ||
| CYP3A4-1942 for | ttttttggtttgatgtttgt | 717 | |
| CYP3A4-1226 rev | actccaataaaataatccca | ||
| CYP3A4-1604 for (nest) | Ggttgaatgtgagaaagaat | 286 | |
| CYP3A4-1319 rev (nest) | Aaaaaacaaaccccaaaaac | ||
| CYP3A7 proximal | CYP3A7-113 for | Aggttgttggattgtttata | 290 |
| CYP3A7-401 rev | Caaccataaaaacaaaaaaaaa | ||
| CYP3A7-122 for (nest) | Gattgtttatatgttggaga | 203 | |
| CYP3A7-324 rev (nest) | Ccttaaactccccaataaca |