TABLE 4

Summary of precursor and product ions observed in high-resolution mass spectra for AMG 416 and its in vivo nonprotein biotransformation products

Component	Observed Precursor Ion  (m/z; +2 Charge State)	Mass Shift^{^a}	Observed Product Ions^{^b}
		Da	m/z
AMG 416	524.770	−0.003	448.276, 464.262, 481.257
M1	505.248	−39.047	448.276, 464.263
M2a	617.288	185.033	448.277, 464.264, 481.256
M2b	617.288	185.033	448.277, 464.262, 481.255
M3	638.808	228.073	448.276, 464.261, 481.257
M4	574.780	100.017	448.276, 464.262, 481.256, 524.771
M5	525.263	0.983	448.768, 464.753, 481.748
M6	671.309	293.075	448.278, 464.272
M7	698.830	348.117	448.277, 465.264, 481.265, 617.806
M8	568.780	88.017	448.277, 464.264, 481.257
M9	560.767	71.991	448.279, 464.265, 481.263
M10	617.804	186.065	448.275, 465.262
M11	465.272	−118.999	448.284
M12	464.764^{^c}	807.498	448.271, 481.255
M13	553.285	57.027	448.278, 465.268
M14	531.777	14.011	448.274, 465.271
M15	589.296	129.049	448.279, 465.269, 481.254, 524.773
M16	643.313	237.083	448.279, 488.262, 634.805
M22	575.774	102.005	448.279, 464.263, 553.778
M28	489.264	−71.015	481.751

↵^a Mass shift is the difference of observed singly charged m/z − calculated AMG 416 singly charged m/z (1,048.536).
↵^b Products of the ¹²C precursor ion except for M28, which was obtained from fragmentation of [¹⁴C]M28.
↵^c +4 charge state.