Experimental conditions
| Condition | pH | NH4Cl Prepulse | Transport Buffer | Transport Buffer Composition (mM) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| NaCl | KCl | KH2PO4 | MgSO4 | CaCl2 | HEPES | Glucose | ||||
| A | 7.4 | Yes | K+-based | — | 130 | 2.0 | 1.2 | 1.0 | 20 | 5.0 |
| B | 7.4 | No | K+-based | — | 130 | 2.0 | 1.2 | 1.0 | 20 | 5.0 |
| C | 7.4 | No | Na+-based | 118 | 4.7 | 1.2 | 1.2 | 1.5 | 25 | 11 |