TABLE 3

Saturation kinetics of multidrug and toxin extrusion (MATE)1- and MATE2-K-mediated uptake of [¹⁴C]metformin, [³H]thiamine, [³H]MPP⁺, [³H]E3S, and rhodamine 123

Uptake of [¹⁴C]metformin (10–10,000 μM, 1 min), [³H]thiamine (0.1–1000 μM, 1 min), [³H]MPP⁺ (0.1–1000 μM, 1 min), [³H]E3S (0.1–300 μM, 2 min),and rhodamine 123 (0.1–100 μM, 2 min) was determined at condition A (see Table 1). Kinetic parameters were estimated by nonlinear regression analysis and are given as mean ± S.D. from one experiment in case of [¹⁴C]metformin and [³H]MPP⁺ and from one representative experiment of at least two separate experiments in case of the other substrates.