Primer sequences for quantitative real-time PCR
Primers were designed to amplify either all transcription variants or variants that predominate in the liver.
| Gene | Accession No. | Forward Primer (5′–3′) | Reverse Primer (5′–3′) |
|---|---|---|---|
| CYP2C19 | NM_000769.2 | GAAAAATTGAATGAAAACATCAGGATTG | CGAGGGTTGTTGATGTCCATC |
| CAR | NM_005122.4 | GCAGCTGTGGAAATCTGTCA | CAGGTCGGTCAGGAGAGAAG |
| GRα | NM_000176.2 | GGGGAAGAGGGAGATGGA | GTGGTCAGAATGGGAGGC |
| PXR | NM_022002.2 | CAAGCGGAAGAAAAGTGAACG | CTGGTCCTCGATGGGCAAGTC |
| NM_033013.2 | |||
| NM_003889.3 | |||
| GATA-4 | NM_001308094.1 | CTGGCCTGTCATCTCACTACG | GGTCCGTGCAGGAATTTGAGG |
| NM_001308093.1 | |||
| NM_002052.4 | |||
| HNF-3γ | NM_004497.2 | ATGCTGGGCTCAGTGAAGAT | CAGGTAGCAGCCATTCTCAA |
| HNF-4α | NM_178849.2 | GGTCAGGTGGGGTGGATGATATAATG | TCTAGGTTAATAGGGAGGAAGGGAGG |
| NM_178850.2 | |||
| NM_001258355.1 | |||
| GAPDH | NM_002046.5 | GAAGGTGAAGGTCGGAGTC | CAAAGTTGTCATGGATGACC |
| NM_001289745.1 | |||
| NM_001289746.1 |
GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GR, glucocorticoid receptor; HNF, hepatocyte nuclear factor; PXR, pregnane X receptor.