TABLE 1

General reaction conditions for the Vivid P450 inhibition assays

P450 BaculosomesVivid SubstrateCYP450 (nM)Vivid Substrate (µM)
CYP1A2EOMCC53
CYP2C9BOMCC1010
CYP2D6EOMCC1010
CYP3A4BOMCC510
  • BOMCC, 7-benzyloxymethyloxy-3-cyanocoumarin; EOMCC, 7-ethylmethyloxy-3-cyanocoumarin.

  • All assays were performed with 3.33 mM glucose-6-phosphate, 0.3 U/ml glucose-6-phosphate dehydrogenase, and 30 μM NADP+ in potassium phosphate buffer, pH 8.0.