Parameters (unit) | Yeast Transformant | |||
---|---|---|---|---|
pPIC3.5K | pPIC3.5K-hPEPT2 | pPIC3.5K-mPEPT2 | pPIC3.5K-rPEPT2 | |
Vmax (pmol/OD/min) | N.A. | 14.8 ± 0.4A | 1.7 ± 0.1B | 14.8 ± 0.4A |
Km (μM) | N.A. | 150.6 ± 10.3A | 42.8 ± 7.3B | 36.0 ± 3.5B |
Vmax/Km (μl/OD/min) | N.A. | 0.098 ± 0.007A | 0.040 ± 0.007B | 0.411 ± 0.041C |
r2 | 0.998 | 0.997 | 0.974 | 0.992 |
Gene copy numbera | 0.98 ± 0.01 | 0.99 ± 0.01 | 0.98 ± 0.02 | 0.97 ± 0.01 |
N.A., not applicable.
↵a Gene copy number was measured by real-time PCR using human, mouse, and rat PEPT2 primers, and the ARG4 gene as a control; AOX1 gene primers were used for vector alone (Table 3).