TABLE 2

Apparent kinetic parameters of luteolin and Lut-7-G methylation obtained from RLS9

Calculations were based on curve fitting using the Michaelis–Menten equation or biphasic model, as described in Materials and Methods. Data are expressed as the mean ± S.D., n = 3.

MetabolitesKm (μmol/L)Vmax (nmol/mg/min)CLint (mL/min/mg)
LuteolinChrysoeriola33.8 ± 4.11.06 ± 0.050.03 ± 0.01
Diosmetina17.9 ± 1.70.92 ± 0.140.05 ± 0.01
Lut-7-GChr-7-Gb8.9 ± 0.60.38 ± 0.010.04 ± 0.01
Dio-7-Gb15.1 ± 1.10.67 ± 0.020.04 ± 0.01
  • a Biphasic equation.

  • b Michaelis–Menten equation.