TABLE 4

Recovery of dose as pefloxacin and metabolites in urine, bile, and feces after a single 25-mg/kg oral dose of [14C]pefloxacin or pefloxacin to male bile-cannulated Sprague-Dawley rats

MethodParent/Metabolite% Dose Eliminated
Urine (0–24)Bile (0–12 h)Feces (0–48 h)Total
Radioactivity profilingParent (pefloxacin)4.941.8712.8619.67
M43.3232.94BQL36.26
M61.180.520.312.01
M7 (norfloxacin)6.090.191.307.58
M85.701.800.137.63
19F NMR profilingParent (pefloxacin)5.189.5015.81b30.49
M43.9316.88BQL20.81
M61.64aBQL1.64
M7 (norfloxacin)6.60BQLc6.60
M86.211.50BQL7.71
  • a Could not be positively identified. Three unidentified peaks detected accounted for 4.24%, 2.51%, and 1.74% of dose.

  • b Value includes M7 (norfloxacin).

  • c Overlapped with pefloxacin and could not be resolved in data analysis by Mnova MNR.